This book presents the state-of-art marine metagenome research and explains the method of marine metagenomic analysis in an easy-to-understand manner.
Changes in the marine environment due to global warming and pollution have become a major global problem. Maintaining a healthy marine ecosystem requires advanced environmental monitoring and assessment systems. As such, the book presents a novel metagenomic monitoring method, which has been developed for comprehensive analyses of the DNA of microorganisms living in seawater to further our understanding of the dynamics of the marine environment. The book can be used as a primer for new researchers and as a manual on experimental methods.

Metagenomic Methods: From Seawater to the Database (Md. Shaheed Reza, Atsushi Kobiyama, Jonaira Rashid, Yuichiro Yamada, Yuri Ikeda, Daisuke Ikeda et al.)....Pages 3-16
Collection of Microbial DNA from Marine Sediments (Tomoko Sakami)....Pages 17-20
Primer Design, Evaluation of Primer Universality, and Estimation of Identification Power of Amplicon Sequences In Silico (Akifumi S. Tanabe, Satoshi Nagai, Yuki Hongo, Motoshige Yasuike, Yoji Nakamura, Atushi Fujiwara et al.)....Pages 21-36
High Coverage Expression Profiling (HiCEP) of Microbial Community Genomes in the Ocean (Reiko Fujimura, Harunobu Yunokawa, Koji Hamasaki)....Pages 37-50
Front Matter ....Pages 51-51
The Application of DDCA to Metagenomic Analysis (Kazutoshi Yoshitake, Kyohei Matsuno, Atsumi Tsujimoto)....Pages 53-63
Horizontal Gene Transfer in Marine Environment: A Technical Perspective on Metagenomics (Yoji Nakamura)....Pages 65-84
MAPLE Enables Functional Assessment of Microbiota in Various Environments (Hideto Takami)....Pages 85-119
Front Matter ....Pages 121-121
Comparison of Microscopic and PCR Amplicon and Shotgun Metagenomic Approaches Applied to Marine Diatom Communities (Tsuyoshi Watanabe, Tomoko Sakami)....Pages 123-136
Seasonal Dynamics of Bacterial Community Composition in Coastal Seawater at Sendai Bay, Japan (Tomoko Sakami, Tsuyoshi Watanabe, Shigeho Kakehi)....Pages 137-147
Shotgun Metagenome Analyses: Seasonality Monitoring in Sendai Bay and Search for Red Tide Marker Sequences (Kaoru Matsumoto, Norikazu Kitamura, Kazuho Ikeo)....Pages 149-159
Distribution and Community Composition of Ammonia-Oxidizing Archaea and Bacteria in Coastal Sediments in Response to Sediment Material Gradients at Sendai Bay, Japan (Tomoko Sakami, Shigeho Kakehi)....Pages 161-181
Marine Metagenomic Sequence Counts of Reads Assigned to Taxa Consistently Proportionate to Read Counts Obtained for per g of Seawater Sample (Toshiaki Kudo, Md. Shaheed Reza, Atsushi Kobiyama, Jonaira Rashid, Yuichiro Yamada, Yuri Ikeda et al.)....Pages 183-188
New Aquaculture Technology Based on Host-Symbiotic Co-metabolism (Miyuki Mekuchi, Taiga Asakura, Jun Kikuchi)....Pages 189-228
Front Matter ....Pages 229-229
Influences of Diurnal Sampling Bias on Fixed-Point Monitoring of Plankton Biodiversity Determined Using a Massively Parallel Sequencing-Based Technique (Satoshi Nagai, Noriko Nishi, Shingo Urushizaki, Goh Onitsuka, Motoshige Yasuike, Yoji Nakamura et al.)....Pages 231-248
Mining of Knowledge Related to Factors Involved in the Aberrant Growth of Plankton (Yasuhito Asano, Hiroshi Oikawa, Motoshige Yasuike, Yoji Nakamura, Atushi Fujiwara, Keigo Yamamoto et al.)....Pages 249-271

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