Protocols for Macroalgae Research describes state-of-the-art approaches and applications in seaweed research. The book is comprised of 31 protocols in all and provides critical information regarding the cultivation and preservation of seaweeds, their chemical composition, as well as their cellular and molecular characterization. This type of research is critical and timely, as the last few decades have witnessed remarkable advancements in phycological research worldwide. Consideration of algal resources for food, fuel, chemicals, feed, and fertilizer has furthered the development of diverse laboratory techniques that not only advance their utilization substantially, but also provided new insights in understanding the molecular basis of their form and function. This recent progress has enabled researchers to determine and quantify various metabolites and organic macromolecules such as carbohydrates, proteins, and lipids.



Protocols for Macroalgae Research thus uses macroalgae as a model organism for addressing fundamental research problems. No recent comprehensive literature of this format exists on this topic. As such, this state-of-the-art protocol text both develops and standardizes the various macroalgae approaches located within the field. It does so by featuring a wide-range of protocols from invited experts; each protocol features a brief introductory overview, an inventory of materials and reagents, and step-by-step experimental procedures, which include helpful notes offering care and cautions. This protocol book is an invaluable reference for beginning and preexisting researchers within the field of macroalgae.

Section I: Cultivating and preserving seaweeds
Chapter 1: Seaweed in high-energy environments: Protocol to move Saccharina cultivation offshore
Chapter 2: Cultivation protocol for Saccharina latissima
Chapter 3: Derivation of clonal stock cultures and hybridization of kelps: A tool for strain preservation and breeding programs
Chapter 4: Cryopreservation of macroalgae
Chapter 5: Unraveling seaweeds bacteriomes: From field site to computer screen Chapter 6: Heavy metal ecotoxicity on the early life history stages of macroalgaeChapter 7: A simple protocol for a rapid and consistent production of a large number of viable protoplasts from the Ulvophycean species
Chapter 8: Purification of sporulation and swarming inhibitors from Ulva: Application in algal life-cycle controlling
Chapter 9: Preparation of axenic cultures in Ulva (Chlorophyta)
Section II: Chemical composition
Chapter 10: Biochar production from seaweeds
Chapter 11: Identification and quantification of laminarins in brown algae Chapter 12: Determination of carbohydrate composition of macroalgaeChapter 13: Quantification of proteins in seaweeds
Chapter 14: Comprehensive phytohormone quantification in the red alga Pyropia yezoensis by liquid chromatography-mass spectrometry
Chapter 15: Total phenolic content and antioxidant capacity analysis of seaweed biomass
Chapter 16: Extraction of phycocyanin and phycoerythrin pigments
Chapter 17: Quantification and localization of reactive oxygen species in marine macrophytes Chapter 18: Metabolomics of intra- and extracellular metabolites from micro- and macroalgae using GC-MS and LC-MSChapter 19: Preparative extraction of exometabolites from seaweed surfaces
Chapter 20: Disruption-free solid-phase extraction of surface metabolites from macroalgae
Section III: Cellular and molecular characterization
Chapter 21: The immunodetection and in situ imaging of cell-wall polysaccharides in brown algae
Chapter 22: Atomic force microscopy based analysis of cell-wall elasticity in macroalgae Chapter 23: Dynamic and microscale mapping of cell growth: Case of Ectocarpus filament cellsChapter 24: Actin fluorescent staining in the filamentous brown alga Ectocarpus siliculosus
Chapter 25: Cryofixation of brown algae for transmission electron microscopy
Chapter 26: Probing the subcellular topography of seaweeds: Transmission electron microscopy, immunocytochemistry, and correlative light microscopy
Chapter 27: Coralline algae preparation for scanning electron microscopy and optical microscopy
Chapter 28: Extraction of high quality RNA from brown algae for transcriptomic analysis